Symptoms of the Life Changing, Psychoanalytical Phenomenon Essay

She rolls over, still completely exhausted from the minimal forty- five-minute sleep, and hits the snooze on the alarm. She can hardly force her jelly-like legs to the chilled floor. Eyes slightly cracked, open enough to see shapes of objects, she throws on the first clothing items she finds on her floor. Breakfast sounds revolting, but she knows she will need something to keep her going through the day, having been awake until six A.M. with thoughts of worry and obsession over problems the day is sure to bring. As she climbs into her car, she hardly notices her jacket is not zipped in the brisk winter air, and should not be driving from lack of concentration. School is the last place she would prefer to be; with no homework done, she begins to worry again about her achievements she has been working towards. Upon arriving to the first class of the day, her eyes are already shutting as if they were automatic doors, and the stress of the babbled lecture tunes her out. After hard ly making it through the day, she crawls into her bed with mountainous amounts of homework. Not caring, or able to motivate herself, she ignores the learning she was once passionate for, therefore, starting the cycle over for the following day. Does this sound familiar? What is being experienced by the student above is generalized anxiety disorder, or GAD, and is also experienced by 3.1 percent of the United States population (â€Å"Generalized Anxiety Disorder† 1). This is an example of one of the various types of the growing issue of anxiety amongst humanity. Anxiety can appear in people of all genres, and also takes a varying life within each individual stricken with this mental illness. Though seemingly helpless, there are vast amounts of treatment ... ...n be sought, both seeking professional help as well as oneself. One can turn to therapy, both behavior changing and thought breaking, turning negativity in to positive life living. Along with therapy, medications work wonders on an anxious mind, as well as self help solutions. In all, anxiety should not be taken lightly and needs instant attention to avoid further self-harm. Works Cited Bloomfield, Harold H. M.D. â€Å"Coping With Anxiety- Worry Free Life.† New York: Harper Collins. 1998. Web. 6 Dec. 2010. Comer, Ronald J. â€Å"Fundamentals of Abnormal Psychology.† 2011, 2008, 2005 by Worth Publishers. â€Å"Generalized Anxiety Disorder.† Anxiety Disorders Association of America. Web. 6 Dec 2010. Smith, Melinda. â€Å"Anxiety Attacks and Disorders.† November 2010. Web. 6 Dec. 2010. â€Å"Stress and Anxiety.† New York Times. Web. 6 Dec. 2010.New York Times. Web. 6 Dec. 2010.

California v Carney :: essays research papers

California v. Carney involves a Drug Enforcement Agency Agent, Robert Williams, who was observing respondent, Charles Carney, as he approached a youth in downtown San Diego. Having received previous information that that particular motor home was being used to exchange sex for marijuana, Williams accompanied by other agents kept the motor home under surveillance (Kamisar, LaFave, Israel, King, p 260, 2002). During the time that the agent had Carney under surveillance, he saw Carney bring the youth back to his motor home, which was parked in a lot (Kamisar, et al., p 260, 2002).   Ã‚  Ã‚  Ã‚  Ã‚  After approximately an hour and fifteen minutes the youth exited the motor home. The youth was then stopped by the agents who engaged him in conversation. At this point the youth told the agents that he had received marijuana in return for allowing Carney sexual contact (Kamisar, et al., p 260, 2002). Cooperating with the agents, the youth returned to the motor home and knocked on the door. When the respondent stepped out one of the agents entered the home without having a warrant or any form of consent. Inside the agent saw marijuana, and a following investigation at the precinct exposed more marijuana. Charles Carney was charged with ownership of marijuana for sale (Kamisar, et al., p 260, 2002).   Ã‚  Ã‚  Ã‚  Ã‚  In California v. Carney, the California Supreme Court questioned if the warrant less search of the respondent’s motor home violate his Fourth Amendments right to privacy. After his motion to exclude the evidence found in the motor home was denied, the California Supreme Court held that the search of the motor home was unreasonable and that the motor vehicle exception to the warrant requirement of the Fourth Amendment did not apply to this case. They reasoned that the expectations of privacy in a motor home are more reminiscent of those in a private residence than in a vehicle (Kamisar, et al., p 260-61, 2002).   Ã‚  Ã‚  Ã‚  Ã‚  The court was able to some to this conclusion based on the idea that the â€Å"expectations of privacy in a motor home are more like those of a home rather than a car because the most important function of motor homes is not to endow with transportation but to provide the occupant with living quarters† (Kamisar, et al., p 261-62, 2002). The Fourth Amendment protects the â€Å"right of the people to be secure on their persons, houses, papers, and effects, against unreasonable searches and seizures† (Gpo.

Child Abuse and Neglect Essay -- essays research papers fc

â€Å"Child Abuse†   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚     Ã‚  Ã‚  Ã‚  Ã‚  Child abuse is a very serious and controversial issue that is escalating in today’s society. As we look back to the 1940’s and 1950’s, it was almost unheard of to let anyone outside of your immediate family know anything about your personal life. Every family was thought to be, or shown to the general public to be, the â€Å"perfect family†. Some schools had the authority to punish a child by either spanking them with a paddle or hitting their knuckles with a ruler. Child abuse is one of the leading causes of childhood deaths in this nation. Statistics show â€Å"every 4 seconds a child is abused and every 7 minutes a child's life is lost† (Neglect 1). We as a society need to work together to try to think of alternative ways of disciplining our children. A child is a gift from God and should be treated as such. I do understand that with the hectic life styles of today’s society, sometimes it just seems like life is a struggle and just too much. Spanking a child as corrective action is one thing, but beating a child, ripping his or her hair out, locking him or her in a closet, or verbally abusing a child is unacceptable. One solution we can consider is...

Micro-Environment Essay

1.0Background Information With the advent of gene technology, it is important to understand not only the phenotype of the organism but also the genotype. Previously, you should have learnt the analysis of genetic traits and the various ways where they can be transmitted from parents to children (by phenotype analysis). Each chromosome is divided into different sections called genes. Genes are the basis of inheritance where traits like hair colour and blood type are controlled by the production of proteins by these genes. Genes contain coded instructions that the body uses to assemble hundreds of different types of proteins that make an individual unique! These amazing trait controllers (genes) are made up of molecules called deoxyribonucleic acid (DNA). DNA is a double-helical polymer bound together by hydrogen bonds between complementary base pairing nucleotides (A to T, G to C). A particular gene is a set of coded instructions made up of a particular order of nucleotides. The variation of which allows the myriad of codes to exist in an organism for it to be unique. This is what controls the genotype of an organism and henceforth, the extraction and isolation of an organisms DNA is imperative, in order to allow further insight into the organism using different molecular-based methods. In this experiment, you will be taking a closer look at this DNA molecule. You will be extracting your own DNA using buccal/cheek cells as the starting material. 2.1Materials Saline 15 ml centrifuge tube Paper cup Drinking water Vortex Centrifuge 10% SDS Bromelain protease (50mg/mL) Ice cold isopropanol Graduated pipettes 2.2Methods IMPORTANT NOTE: Ensure that you have not eaten in the past 1 hour before conducting this experiment (if you are the DNA donor). Ensure that gloves are worn at all times in the experiment. 1. Swish you mouth with about 100 mL drinking water, for about 20 seconds, to remove any food particles. Discard this wash into the sink. 2. Using a permanent marker pen, label your group name onto the paper cup and 15 mL centrifuge tube containing 10mL saline. 3. Pour all the 10mL saline solution into your mouth and vigorously swish for 60s. Do not discard the centrifuge tube. 4. Expel the saline mouthwash into the labelled paper cup. 5. Carefully, pour the saline mouthwash from the paper cup, back into the 15 mL centrifuge tube from step 2. Tightly cap the tube. 6. Pass the capped tubes to the laboratory technician in order to be centrifuged (4500 rpm, 5 min). 7. Upon centrifuging, you should be able to see your buccal cell pellet (the whitish lower solid layer at the bottom of the tube). Gently, pour away the supernatant (the liquid upper layer). 8. Place the tube on ice. 9. Add 2 mL saline into the tube and vortex for 5-10 seconds. 10. Add 1 mL 10% (w/v) sodium dodecylsulphate (SDS) solution (active component in detergents). 11. Gently tap the tubes several times (~8 times) to gently mix the contents. You may invert the tube twice if needed. 12. On ice, add 2 – 3 drops of the lab supplied bromelain protease enzyme into the tube. 13. Gently tap the tubes several times (~8 times) to gently mix the contents. You may invert the tube twice if needed. 14. Cap the tube and place it is a 50oC for 10 minutes. 15. With a clean pipette, gently pipette in 10 mL ice cold isopropanol (95% v/v) slowly into the tube. Tip: Place the filled pipette with its tip against the inside wall of the test tube. Slowly allow the isopropanol to dribble down the inside of the tube. 16. Cap and place the tube in a test tube rack at room temperature for 10 minutes. DO NOT mix, shake, or bump the test tube during this period. 17. The isopropanol is lighter than the contents of the tube. When added according to the directions, the isopropanol will form a clear layer ABOVE the suspension. 18. Observe the test tube for 5 minutes. The DNA will gradually separate from the suspension and rise into the isopropanol layer. Describe the appearance of the DNA. 19. Take a photo as proof of your observation. 20. To remove the accumulated DNA from the tube, follow the directions for DNA spooling as below:- a. Gently insert the glass rod through the isopropanol layer into the clumped/accumulated DNA. b. Carefully, twirl the rod between your fingers, winding the DNA strands onto the rod. c. Slowly remove the rod. Describe the appearance of the spooled DNA. d. Take a photo as proof of your observation. 0. Questions 1. Which one of the following do you think will contain DNA? Explain your reasoning. Bananas; concrete; fossils; meat; metal; spinach; strawberries. 2. What effect would the SDS have on the cell membranes and cold ethanol on DNA? 3. What type of enzyme would be needed to separate the DNA into smaller pieces? 4. Is the DNA extracted pure enough for further applications (i.e. PCR)? 5. If you were to repeat the experiment with an equal number of red blood cells, the amount of DNA collected would either: increase / decrease / stay the same (choose one). Explain your answer. Adapted from:- Bres, M., Weisshaar, A., 2008. Thinking about Biology: An Introductory Laboratory Manual. 3rd Ed. Pearson Prentice Hall: New Jersey, USA. Pg. 333 – 338. Teaching AS Biology Practical Skills. University of Cambridge: International Examination. Pg. 74 – 78. 43 Practical 10 Digestion of Lambda (ÃŽ ») DNA with a Restriction Enzyme (EcoR I endonuclease) 1.0Background Information Restriction enzymes (nucleases) are enzymes that cleave the phosphodiester bonds on the sides of deoxyribonucleic acid (DNA). These nucleases recognize specific DNA sequences in the double-stranded DNA, which is usually a four to six base pair sequence of nucleotides, and digests the DNA at these sites, resulting in the DNA becoming fragmented into various lengths. Some restriction enzymes cut cleanly through the DNA double helix while some produce uneven or sticky ends. By using the same restriction enzyme to cut DNA from different organisms, the sticky ends produced will be complementary and the DNA from the two different sources can be recombined. In humans, no two individuals have the exact same restriction enzyme pattern in the DNA except for identical twins. Restriction enzymes are named based on a system of nomenclature where the first letters represents the genus name of the organism whereas the next two letters come from the species name. If there is a fourth letter, it stands for the strain of the organism. Finally, if there are Roman numerals, it represents whether that particular enzyme was the first or second etc. isolated in that category. FIGURE 10.1 Cartoon of how EcoR I recognises the restriction site and cleaves the DNA. The second technique used in this practical is the separation and analysis of DNA fragments. Agarose gels are commonly used for this where the  gels that have been prepared with a suitable nucleic acid stain in it, have wells for the samples of DNA to go into. The agarose gel is covered in a suitable buffer so that the DNA is in a neutral pH solution. That way, the DNA moves one direction because of its charge. Since the phosphate groups on the skeleton of DNA are negatively charged, the whole molecule takes on the negative charge. Hence, when the DNA is placed inside the gel and the electricity is turned on, the poles are drawing the DNA toward the positive side, where it will then move through the gel and separate according to the size of the fragments. This technique is called electrophoresis. Results are obtained with the help of UV light that is refracted by the nucleic acid stain that sticks onto the DNA fragments. In this experiment, you will be using the EcoR I restriction endonuclease to digest a known DNA called phage lambda (ÃŽ ») and analysing your sample using agarose gel electrophoresis. 2.1Materials Pre-laboratory work Computer/laptop LambdaDNA.docx (Word document file) Laboratory work Micropipette Sterile pipette tips Microcentrifuge tube (1.5 mL capacity) EcoR I (20 U/ µL) and buffer Distilled deionised sterilised water Agarose gel (prestained with EtBr) 1x TAE buffer Agarose gel electrophoresis set UV Transilluminator

Policy Analysis Paper/CJA/464 Essay

Policy is a large part of policing in the United States. Policy helps set law, and law helps keep order amid the chaos if enforced properly. But every policy must be looked at carefully before, during and after enforcement to make sure that policy is and continues to be the best fit for the issues at hand. Sometimes looking at two policies and comparing them can help find a solution in the middle. It is important to give a policy a realistic goal, to accomplish in a realistic time in order to find out if it was truly effective or not. Crime Control Model/ Due Process Model: Crime control model places emphasis on using more police and harder court sentences to help deter and hopefully reduce crime. It utilizes the powers held by the government to protect the people, with limited regards to their rights (Sociology Index, 2013). It favors tougher punishment to crimes and on the criminals that perform them. Crime Control model argues that sometimes, persons should give up their rights, to protect and benefit society as a whole. Often people are assumed to be guilty until proven innocent. Due Process tries to focus on the rights of the individuals and limiting the power held by the government. People that wish to limit government tend to favor the due process model over the crime control model. In the due process model, people are innocent until proven guilty, and are not adequately punished until their guilt is well established through the criminal justice system. Each model has their place in our criminal justice system, having both strengths and weaknesses. As society changes, the models must be willing to trade off to adapt to the crime conditions for that time. Both embrace some aspects of the core values of the constitution, and while the ways they can work together are few, they do have their moments. Policies/ Opinion: The Border Search of Information Policy is a policy set forth in 2008 which allows Border Patrol agents the ability to search and seize both printed and electronic materials and devices without needing a warrant or probable cause (CPB, 2008). If an individual is going through a check point or any Border Patrol station, the officers may choose to pull them aside for a random inspection. During the course of that inspection they may go through any and all electronic and printed items (ACLU, 2013). ICE and Department of Homeland Security have similar policies. These policies all seem to throw out the fourth amendment which states â€Å"The right of the people to be secure in their persons, houses, papers, and effects, against unreasonable searches and seizures, shall not be violated, and no Warrants shall issue, but upon probable cause, supported by Oath or affirmation, and particularly describing the place to be searched, and the persons or things to be seized†. (Fourth Amendment, 1789) Some argue that this is a necessary right to forgo, to stop such things as child pornography, or links to terrorism (DHS, 2014). I have even heard the excuse of â€Å"If you have nothing to hide, it shouldn’t be a problem. † I respectfully have to disagree, quoting Benjamin Franklin â€Å"They who would give up essential Liberty, to purchase a little temporary Safety, deserve neither Liberty nor Safety. † (Franklin). Currently this policy seems to favor crime control, and not due process, violating people’s rights without just cause. Roles of the Courts: The courts play a few roles in this policy. One role is as they are prosecuting the violations several cases might get thrown out due to the constitutionality of the search. Courts have the power to look at this policy, and try to find a way to fix the constitutional rights that are being violated within before people that deserve to get punished, get set free to continue their illegal activities. If this does not change, many more innocent people maybe hurt as their rights are violated trying to attempt to catch people that will try to get away with a crime any way they can. Some may think they have nothing to hide, that they’ve done nothing wrong, only to be caught because in a text message they talk about something private that may raise questions in the eyes of the officer. Conclusion: . The people need to know that they are going to be innocent until proven guilty and they have to trust that the government always has their best interest in mind. This includes keeping their essential constitutional rights in mind, and protecting them from possible abuse of power by officers who feel above the law. Due process and Crime control models do have their place, and the country’s courts must attempt to find a balance between the rights of the individuals and the protection of society. By analyzing the policies at hand, and establishing clear boundaries ensures that the criminals are caught the correct way, and the rights of the innocent people are left undamaged.

Demat account Essay

The trading on stock exchanges in India used to take place through open outcry without use of information technology for immediate matching or recording of trades. This was time consuming and inefficient. This imposed limits on trading volumes and efficiency. In order to provide efficiency, liquidity and transparency, NSE introduced a nation-wide on-line fully automated screen based trading system where a member can punch into the computer quantities of securities and the prices at which he likes to transact and the transaction is executed as soon as it finds a matching sale or buy order from a counter party. Screen based electronic system electronically matches orders on a strict price/time priority and hence cuts down on time, cost and risk of error, as well as on fraud resulting in improved operational efficiency. It allows faster incorporation of price sensitive information into prevailing prices, thus increasing the informational efficiency of markets. It enables market participants, irrespective of their geographical locations, to trade with one another simultaneous, improving the depth and liquidity of the market. It provides full anonymity by accepting orders, big or small, from members without revealing their identity, thus providing equal access to everybody. It also provides a perfect audit trial, which helps to resolve disputes by logging in the trade execution process entirety. The sucked liquidity from other exchanges and in the very first year of its operation, NSE became the leading stock exchange in the country, impacting the fortunes of other exchanges and forcing them to adopt SBTS also. Today India can boast that almost 100% trading take place through electronic order matching. In order to promote dematerialization of securities, NSE joined hands with leading financial institutions to establish the national securities depository Ltd. (NSDL), the first depository in the country, with the objective of enhancing the efficiency in settlement systems as also to reduce the menace of fake/forged and stolen securities. This has ushered in an era of dematerialised trading and settlement. SEBI has made dematerialised settlement mandatory in an ever -increasing number of securities in a phased manner, thus bringing about an increase in the proportion of shares delivered in dematerialised form. There is an increasing preference to settle trades, particularly in high value securities, in demat form. Such  high level of demat settlement reassures success of rolling settlement. What is DEMAT In India , a demat account, the abbreviation for dematerialised account, is a type of banking account which dematerializes paper-based physical stock shares. Conversion of Securities from Physical (Paper) Mode into Electronic Mode is Called Dematerialisation. The Client opens Demat Account with any DP. Upon Demat, the Certificates are destroyed and credit entry of exactly equal number of Securities is created in Depository in Electronic mode. The B.O. account of holder is credited and the securities loose their identities. The dematerialised account is used to avoid holding physical shares: the shares are bought and sold through a stock broker. This account is popular in India. The market regulator, securities and exchange board of India (SEBI) mandates a demat account for share trading above 500 shares. As of April 2006, it became mandatory that any person holding a demat account should possess a permanent account number (PAN), and the deadline for submission of PAN details to the depository lapsed on January 2007. Dematerialisation is the process by which physical certificates of an investor are converted to an equivalent number of securities in electronic form and credited in the investor’s account with its DP. In order to dematerialize certificates; an investor will have to first open an account with a DP and then request for the dematerialisation of certificates by filling up a dematerialisation request form [DRF], which is available with the DP and submitting the same along with the physical certificates. The investor has to ensure that before the certificates are handed over to the DP for demat, they are defaced by marking â€Å"Surrendered f or Dematerialisation† on the face of the certificates. Objective of the Study †¢ The main objective of the study is to know about the potential of the market regarding people’s dealing in share market. †¢ To know the role of Demat Account . †¢ To know the procedure of opening DEMAT ACCOUNT. †¢ The objective is to know that how many people in the city are aware of the UNICON SECURITIES PVT. LTD. †¢ To know where people have already opened their demat a/c and on what basis. Procedure for Opening an Account A demat account are opened on the same lines as that of a Bank Account. Prescribed Account opening forms are available with the DP, needs to be filled in. Standard Agreements are to be signed by the Client and the DP, which details the rights and obligations of both parties †¢ The DP officials will make available the relevant account opening form (depending on whether the client is a retail investor or corporate client/clearing member) and specify the list of documents regarding references that should be submitted along with the form. It will also give a copy of the relevant agreement to be entered with the client, in duplicate. †¢ The client will submit the duly filled in account opening form and client has to visit personally for opening the account in DP. The DP officials have to do in person verification and affix the â€Å"IN PERSON VERIFICATION† stamp on the account opening form. It should also furnish such documents regarding references, as specified by the DP , along with the account opening form. After executing the agreement the client has to forward it to the DP. †¢ The DP officials will verify that the account opening form is duly filled in. It will also verify the enclosed documents, if any. Incomplete forms will be forwarded to the client for rectification. For Corporate Clients, the DP officials will verify if the board resolution for the authorized signatories is enclosed. †¢ In case the documents are not proper, the DP officials will reject the form and intimate the client of the same, stating the reasons for doing so. †¢ If the form is in order; the DP officials will accept the same and affix the stamp â€Å"verified with original† on each and every proof after seeing the original proofs. †¢ After completion of all documentation, the DP officials will verify the pan from income tax website. And affix the stamp â€Å"PAN VERIFIED† with date and sign on the stamp. †¢ The DP officials will enter the client details as mentioned in the account opening form in the DPM (software provided by NSDL & CDSL to the Participant) screen provided for the purpose. In case of NSDL A/c opening the SR. Assist will capture all the details in the DPM and record the client’s signature (on the form) as specimen for authorizations in the future. †¢ After entering client details in the system, a client account number will be generated by the DPM. The DP officials will enter this in the account opening form. After that the officers will verify the details in the DPM captured by the SR. Assist. and  activate the account. †¢ The DP officials are not allowed to give the demat a/c no to the clients until the a/c is activate, this is applicable for both NSDL & CDSL. †¢ When the demat a/c is activated the DP officials have to send the client master and the copy of agreement between DP and client at the client’s address DEMAT Process †¢ The registered holder of the securities makes the request. †¢ Securities must be recognised by Depository as eligible. †¢ Client submits DRF & physical certificates to DP. DP checks securities. †¢ Client defaces certificates and DP punches two holes on name of company. †¢ DP enters demat request in system for Depository. †¢ DP dispatches certificates along with DRF to R&T. †¢ Depository records the details and sends to R&T. †¢ R&T agent verifies the details and confirms to Depository. †¢ Depository credits the demat securities to BO a/c of client and intimates DP electronically. †¢ DP issues statement to client

Effects of CClF3 on Ozone

CC13F to enter the stratosphere (1930 to 1955). 2. The ozone layer above Canada began to decline in 1975. 3. Once the production of CC13F was drastically reduced, it took 20 years for the CC13F levels to decrease in the stratosphere. Conclude and Communicate 4. CFCs (Chlorofluorocarbons) are inorganic man-made chemicals that have been in production for more than 50 years in Canada.They were thought to be sensational substances due to their stability, nonflammable characteristic, low in toxicity, and inexpensive. However, research on CFCs showed that they have long life spans allowing them to resist being washed away in rain. Through the aid of wind, CFCs rise up into the stratosphere and into the ozone layer where they decompose into chlorine and bromine, from the ultraviolet radiation. These two chemicals are responsible for damaging the ozone layer.Some atmospheric chlorine are caused by natural occurrences such as large fires and volcanic eruptions, yet most chlorine in the ozone layer is due to CFCs from man-made products such as refrigerators, aerosols, solvents and other household items. Studies show that for every one hlorine atom that is released into the ozone layer, 100000 ozone molecules are destroyed. According to the statistics recorded in 1979, the ozone layer has decreased every single decade nearly 4% to 6% in mid-latitudes and 10% to 12% in higher southern latitudes.This constant decrease has left the ozone layer permanently damaged. Even though production of CFCs has drastically decreased over the past couple of years, the ozone layer is unable replace itself. Research on how Earth is affected by the depletion in the ozone layer 5. CFCs are so stable that the only way to break them is by exposure to strong UV radiation. When this occurs, the CFC molecule releases chlorine that can destroy 100000 ozone molecules.